Stimulatory and Inhibitory Effects of Low and High Concentrations of Genistein on Human Hepatocellular Carcinoma HepG2 Cell Line

Masumeh Sanaei, Fraidoon Kavoosi

Abstract


Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. It is a fatal disease with a high incidence in sub-Saharan Africa, China, and the Far East and a low incidence in the United States and Europe. The major recognized risk factors for the disease are viral, toxic, metabolic and immune-related. Soy isoflavones have been identified as dietary components having an important role in reducing the incidence of cancers. Genistein (GE), the predominant isoflavone found in soy products, has biphasic effects. It has stimulatory or inhibitory effects according to concentration. The aim of the present study was to analyze the stimulatory and inhibitory effects of genistein on hepatocellular carcinoma HepG2 cell line. Materials and Methods: Cells were treated with various concentrations of genistein and the MTT assay was used and then cells were treated with single dose of genistein (20μM) and flow cytometry assay was performed. Results: genistein indicated stimulatory and inhibitory effects according to concentration.  Discussion: Our finding clearly indicated that genistein has a significant inhibitory and stimulatory effects related to concentration. Conclusion: genistein can play biphasic role on the growth of HCC cells.


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