Effect of 17-β estradiol on Proliferation and Apoptosis of Hepatocellular Carcinoma HepG 2 Cell Line
Phytoestrogens are a group of natural compound with estrogen-like activity and similar structure of estradiol, biologicall active phenolic compounds that structurally mimic the mammalian estrogen 17-betaestradiol. Numerous epidemiological studies suggest that diets rich in phytoestrogens, particularly soy and unrefined grain products, may be associated with low risk of some cancers. Phytoestrogens induce apoptosis at high concentrations and also consumption of soy products is associated with a decreased risk of cancer. The aim of the present study was to analyze the effect of 17-betaestradiol on proliferation and apoptosis of hepatocellular carcinoma HepG 2 cell line. Materials and Methods: MTT assay and flow cytometry assay were used to evaluate proliferative and apoptotic effects of 17-betaestradiol. Results: 17-betaestradiol inhibited the growth of HepG 2 cell and induced apoptosis significantly with a time- and dose-dependent manner. Discussion: Our finding clearly indicated that 17-betaestradiol has a significant inhibitory and apoptotic effects. Conclusion: 17-betaestradiol can significantly inhibit the growth and induce apoptosis in the HepG 2 cell line.
Stefan O. Mueller, Stephanie Simon, Kun Chae, et al. Phytoestrogens and Their Human Metabolites Show Distinct Agonistic and Antagonistic Properties on Estrogen Receptor a (ERa) and ERb in Human Cells. Toxicological science. 2004; 80: 14–25
Ashish Kumar Sharma, Vipin Agarwal1, Bhupendra Chauhan,et al. Role of phytoestrogen in treatment of cancer. International Journal of Pharma Research and Development. 2010; 2 (9): 22-32
Peeters PH, Keinan-Boker L, van der Schouw YT et al. Phytoestrogens and breast cancer risk. Review of the epidemiological evidence. Breast Cancer Res Treat. 2003; 77: 171-183
Lee MM, Gomez SL, Chang JS, et al. Soy and isoflavone consumption in relation to prostate cancer risk in China. Cancer Epidemiol Biomarkers Prev. 2003; 12: 665-668
Morton MS, Arisaka O, Miyake N, et al. Phytoestrogen concentrations in serum from Japanese men and women over forty years of age. J Nutr. 2002; 132: 3168–3171.
Adlercreutz H, Honjo H, Higashi A, et al. Urinary excretion of lignans and isoflavonoid phytoestrogens in Japanese men and women consuming a traditional Japanese diet. Am J Clin Nutr 1991; 54: 1093–1100.
Wu AH, Yu MC, Tseng CC, et al. Plasma isoflavone levels versus self-reported soy isoflavone levels in Asian-American women in Los Angeles County. Carcinogenesis. 2004; 25: 77–81.
Wu AH, Yu MC, Tseng CC, et al. Epidemiology of soy exposures and breast cancer risk. Br J Cancer 2008; 98:9–14.
Thompson LU, Chen JM, Li T, et al. Dietary flaxseed alters tumor biological markers in postmenopausal breast cancer. Clin Cancer Res. 2005;11: 3828–35.
Jane L Limer and Valerie Speirs. Phyto-oestrogens and breast cancer chemoprevention. Breast Cancer Research. 2004; 6:119-125
Lian, F, Li Y, Bhuiyan M et al. p53-independent apoptosis induced by genistein in lung cancer cells. Nutr Cancer. 1999; 33: 125-131
Horn-Ross PL, John EM, Canchola AJ, et al. Phytoestrogen intake and endometrial cancer risk. J Natl Cancer Inst. 2003; 95: 1158-1164
Sarah M. Mense, Tom K. Hei, Ramesh K, et al. Phytoestrogens and Breast Cancer Prevention: Possible Mechanisms of Action. Environ Health Perspect.2008;116: 426–433
M. Nikbakht Dastjerdi, F. kavoosi. Inhibitory effect of 17-beta estradiol on hepatocellular carcinoma cell line. Indian journal of applied research. 2015; 5(1): 435-439
Talitha T. Rajah, Kevin J. Peine. NGA DU, Christina A. Serret et alPhysiological Concentrations of Genistein and 17β-Estradiol Inhibit MDA-MB-231 Breast Cancer Cell Growth by Increasing BAX/BCL-2 and Reducing pERK1/2. Anticancer Research April. 2012 ; 32 ( 4): 1181-1191
ParaskeviMoutsatsou. The spectrum of phytoestrogens in nature: our knowledge is expanding. Hormones 2007, 6(3):173-193
Adlercreutz H. Phytoestrogens and cancer. The Lancet Oncol.2002; 3: 364-373.
Yi-Ting Huang, Tai-Lung Cha, Guang-Huan Sun. Estrogen Inhibits Renal Cell Carcinoma Cell Progression through Estrogen Receptor-β Activation. plos. 2013; Published: 2013; 13: 17-25
LitalKeinan-Boker, Yvonne T van Der Schouw, Diederick E Grobbee, et al. Dietary phytoestrogens and breast cancer risk. Dietary lignans and breast cancer risk. Am J ClinNutr. 2004;79: 282–8.
Myung-Jin Kim, Sung-Il Cho, Kun-Ok Lee1, et al. Effects of 17β-Estradiol and Estrogen Receptor Antagonists on the Proliferation of Gastric Cancer Cell Lines. J Gastric Cancer. 2013; 13(3):172-178.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.
Copyright ©2013 Academic Journals Center
To make sure that you can receive messages from us, please add the 'academicjournalscenter.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.