Effect of Valproic Acid on Proliferation and Apoptosis of Colon Cancer HT 29 Cell Line
The structure of chromatin is made up of DNA, histones, and non-histone proteins with the basic repeating unit, nucleosome. The structure of chromatin can be reversibly modified in several ways including histone acetylation. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are two groups of enzymes that involved in acetylation and regulate gene expression. Histone deacetylases have a key role in the remodeling of chromatin and the epigenetic regulation of gene expression. HDAC inhibitors can inhibit HDACs and affect gene expression. HDAC inhibitor valproic acid (VPA) has antitumor activities against certain cancers that make it an attractive drug for treating cancer. The aim of the present study was to analyze the effect of VPA on proliferation and apoptosisin of colon cancer HT 29 cell line. Materials and Methods: MTT assay and flow cytometry assay were used to evaluate proliferative and apoptotic effects of VPA. Results: VPA inhibited the growth of HT 29 cells and induced apoptosis significantly with a time- and dose-dependent manner. Discussion: Our finding clearly indicated that VPA has a significant inhibitory and apoptotic effects. Conclusion: VPA can significantly inhibit the growth and induce apoptosis in the HT 29 cell line.
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